Imeda Rubashvili
Ivane Javakhishvili Tbilisi State University, Georgia
Title: Development and Validation of Carotenoids Determination Method in Organic Extracts Obtained from Agro Industrial Waste Materials using Extraction Techniques and High Performance Liquid Chromatography
Biography
Biography: Imeda Rubashvili
Abstract
The manufacture of food products and dietary supplements using natural food pigments and other different natural compounds has been attracted attention in the field of modern food industry. One of the necessary and interesting naturals compounds are carotenoids which show strong antioxidant and immunomodulation activities and may prevent degenerative diseases as well. The present research concerns the development and validation of a new, rapid, modern, effective and selective HPLC method for determination of carotenoids – beta-carotene and lycopene in organic extracts obtained from tomato, tangerine and orange agro industrial waste materials using extraction techniques - supercritical fluid and sequential ultrasonication-assisted extractions. The method was developed using RP-18 endcapped LiChroCART 4 x 250 mm, 5 μm column. The method was validated with respect to robustness, system suitability test, specificity, linearity-range, accuracy, precision, limit of detection (LOD) and quantitation (LOQ). The LOD and the LOQ are 0.081µg/mL and 0.041 µg/mL for beta-carotene, 0.034 µg/mL and 0.085 µg/mL for lycopene, respectively. The content of each carotenoid per 1 g of dried local agro industrial waste material varies for beta-carotene 0.290 – 10.030 µg (tomato peel), 0.445 – 3.972 µg (tangerine peel), 0.833 – 2.455 µg (orange peel) and for lycopene 0.418 – 143.79 µg (tomato peel), 0.051 – 179.988 µg (tangerine peel), 0.091 – 0.114 µg (orange peel).